Carbon monoxide form of PEGylated hemoglobin protects myocardium against ischemia/reperfusion injury in diabetic and normal mice

Artif Cells Nanomed Biotechnol. 2013 Dec;41(6):428-36. doi: 10.3109/21691401.2012.762370. Epub 2013 Jan 23.

Abstract

We investigated the pre-clinical utility of carbon monoxide form of PEGylated hemoglobin (PEG-Hb also named SANGUINATE(™)) in myocardial infarction (MI) and in particular the response of diabetic tissues to superimposed ischemia/reperfusion injury. SANGUINATE(™) was evaluated in diabetic and normal mice subjected to 30 min of coronary artery ligation followed by either 48 h or 28 days of reperfusion. Our results demonstrate that SANGUINATE(™) was effective in reducing infarct size when administered either prior to left anterior descending coronary artery (LAD) occlusion or during reperfusion. This finding is an important step in exploring the efficacy of a pharmacoinvasive strategy using SANGUINATE(™) in patients with acute coronary syndromes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomarkers / metabolism
  • Blood Substitutes / chemistry
  • Blood Substitutes / pharmacology
  • Carbon Monoxide / chemistry*
  • Carbon Monoxide / pharmacology*
  • Diabetes Complications / metabolism
  • Diabetes Complications / prevention & control*
  • Energy Metabolism / drug effects
  • Heart / drug effects*
  • Hemoglobins / chemistry*
  • Hemoglobins / pharmacology*
  • Mice
  • Myocardial Infarction / complications
  • Myocardial Infarction / metabolism
  • Myocardial Infarction / prevention & control
  • Myocardial Reperfusion Injury / metabolism
  • Myocardial Reperfusion Injury / prevention & control*
  • Oxidative Stress / drug effects
  • Polyethylene Glycols / chemistry*
  • Polyethylene Glycols / pharmacology*
  • Time Factors

Substances

  • Biomarkers
  • Blood Substitutes
  • Hemoglobins
  • PEG-hemoglobin
  • Polyethylene Glycols
  • Carbon Monoxide