Characteristics of neutralizing antibodies to adenovirus capsid proteins in human and animal sera

Virology. 2013 Mar 15;437(2):118-23. doi: 10.1016/j.virol.2012.12.014. Epub 2013 Jan 23.


Although it is known that Ad5-specific neutralizing antibodies (NAbs) against three major viral capsid components (hexon, penton and fiber) are generated, differences in the frequency and nature of these pre-existing NAbs remain unclear. The results emphasized the contribution of anti-fiber antibodies to Ad5 neutralization responses generated during natural viral infection. Additionally, Ad5-specific NAbs against the fiber knob protein were present in over 90% of the positive serum samples while 42% of the sera had NAbs against hexon in this study based on neutralization assay of anti-HVR and anti-knob subtracted sera and Western blotting analysis. We also found that the trimeric knob was preferentially recognized by fiber-induced NAbs and it was serotype-specific in human adenovirus species C. Results indicated that the trimeric knob protein would be a good candidate antigen for detecting adenovirus serotype-specific NAbs in naturally infected sera.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenoviridae / immunology*
  • Adenoviridae Infections / epidemiology*
  • Adenoviridae Infections / veterinary*
  • Animals
  • Antibodies, Neutralizing / blood*
  • Antibodies, Viral / blood*
  • Antigens, Viral
  • Blotting, Western
  • Capsid Proteins / immunology*
  • Humans
  • Neutralization Tests
  • Seroepidemiologic Studies


  • Antibodies, Neutralizing
  • Antibodies, Viral
  • Antigens, Viral
  • Capsid Proteins