Gastrointestinal tissue diagnosis by laser-induced fluorescence spectroscopy at endoscopy

Gastrointest Endosc. Mar-Apr 1990;36(2):105-11. doi: 10.1016/s0016-5107(90)70961-3.


An endoscope-compatible, optical fiber system has been developed which can be used to obtain laser-induced fluorescence spectra of mucosal abnormalities during endoscopy in real time. The results of our previous in vitro studies have suggested that laser-induced fluorescence tissue spectra are sufficiently unique that they can be used to accurately diagnose mucosal abnormalities in some systems. To test this hypothesis in vivo, laser-induced fluorescence spectra were obtained during colonoscopy from 31 colonic adenomas, 4 hyperplastic polyps, and 32 examples of normal mucosa in 20 patients. The resulting spectra could be used to correctly differentiate adenomas from normal colonic mucosa and hyperplastic polyps in 97% of the specimens studied with the resulting sensitivity, specificity, and positive predictive value of 100%, 97%, and 94%, respectively. These results, although preliminary in nature, suggest that laser-induced fluorescence spectra can be used in the recognition and differential diagnosis of mucosal abnormalities at endoscopy.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenoma / diagnosis*
  • Colonoscopy
  • Diagnosis, Differential
  • Humans
  • Intestinal Neoplasms / diagnosis*
  • Intestinal Polyps / diagnosis*
  • Intestines / pathology*
  • Lasers*
  • Spectrometry, Fluorescence / methods*