TRPM7 mediates breast cancer cell migration and invasion through the MAPK pathway

Xiaojing Meng; Chunqing Cai; Jiguo Wu; Shaoxi Cai; Changsheng Ye; Haiyang Chen; Zhengduo Yang; Hongqiang Zeng; Qiang Shen; Fei Zou

doi:10.1016/j.canlet.2013.01.031

TRPM7 mediates breast cancer cell migration and invasion through the MAPK pathway

Cancer Lett. 2013 Jun 1;333(1):96-102. doi: 10.1016/j.canlet.2013.01.031. Epub 2013 Jan 23.

Authors

Xiaojing Meng¹, Chunqing Cai, Jiguo Wu, Shaoxi Cai, Changsheng Ye, Haiyang Chen, Zhengduo Yang, Hongqiang Zeng, Qiang Shen, Fei Zou

Affiliation

¹ Department of Occupational Health and Occupational Medicine, School of Public Health and Tropical Medicine, Southern Medical University, Guangzhou, China.

PMID: 23353055
DOI: 10.1016/j.canlet.2013.01.031

Abstract

Metastasis is an inherent feature of breast cancer and transient receptor potential (TRP) channels were found to be potentially implicated in this process. Particularly, TRPM7 may regulate cell motility. We therefore examined the expression of TRPM7 mRNA in the Oncomine database and found that TRPM7 is correlated to metastasis and invasive breast cancer. Silencing TRPM7 with RNA interference resulted in a significant decrease in migration and invasion capability of MDA-MB-435 breast cancer cells, and phosphorylation levels of Src and MAPK but not AKT. Our results suggest that TRPM7 regulates migration and invasion of metastatic breast cancer cells via MAPK pathway.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Breast Neoplasms / pathology*
Cell Line, Tumor
Cell Movement
Female
Humans
MAP Kinase Signaling System / physiology*
Neoplasm Invasiveness
Phosphatidylinositol 3-Kinases / physiology
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt / physiology
RNA, Small Interfering / genetics
TRPM Cation Channels / physiology*

Substances

RNA, Small Interfering
TRPM Cation Channels
Protein Serine-Threonine Kinases
Proto-Oncogene Proteins c-akt
TRPM7 protein, human