TRPM7 mediates breast cancer cell migration and invasion through the MAPK pathway

Cancer Lett. 2013 Jun 1;333(1):96-102. doi: 10.1016/j.canlet.2013.01.031. Epub 2013 Jan 23.

Abstract

Metastasis is an inherent feature of breast cancer and transient receptor potential (TRP) channels were found to be potentially implicated in this process. Particularly, TRPM7 may regulate cell motility. We therefore examined the expression of TRPM7 mRNA in the Oncomine database and found that TRPM7 is correlated to metastasis and invasive breast cancer. Silencing TRPM7 with RNA interference resulted in a significant decrease in migration and invasion capability of MDA-MB-435 breast cancer cells, and phosphorylation levels of Src and MAPK but not AKT. Our results suggest that TRPM7 regulates migration and invasion of metastatic breast cancer cells via MAPK pathway.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Breast Neoplasms / pathology*
  • Cell Line, Tumor
  • Cell Movement
  • Female
  • Humans
  • MAP Kinase Signaling System / physiology*
  • Neoplasm Invasiveness
  • Phosphatidylinositol 3-Kinases / physiology
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt / physiology
  • RNA, Small Interfering / genetics
  • TRPM Cation Channels / physiology*

Substances

  • RNA, Small Interfering
  • TRPM Cation Channels
  • Phosphatidylinositol 3-Kinases
  • Protein-Serine-Threonine Kinases
  • Proto-Oncogene Proteins c-akt
  • TRPM7 protein, human