Spatial regulation of VEGF receptor endocytosis in angiogenesis

Nat Cell Biol. 2013 Mar;15(3):249-60. doi: 10.1038/ncb2679. Epub 2013 Jan 27.

Abstract

Activities as diverse as migration, proliferation and patterning occur simultaneously and in a coordinated fashion during tissue morphogenesis. In the growing vasculature, the formation of motile, invasive and filopodia-carrying endothelial sprouts is balanced with the stabilization of blood-transporting vessels. Here, we show that sprouting endothelial cells in the retina have high rates of VEGF uptake, VEGF receptor endocytosis and turnover. These internalization processes are opposed by atypical protein kinase C activity in more stable and mature vessels. aPKC phosphorylates Dab2, a clathrin-associated sorting protein that, together with the transmembrane protein ephrin-B2 and the cell polarity regulator PAR-3, enables VEGF receptor endocytosis and downstream signal transduction. Accordingly, VEGF receptor internalization and the angiogenic growth of vascular beds are defective in loss-of-function mice lacking key components of this regulatory pathway. Our work uncovers how vessel growth is dynamically controlled by local VEGF receptor endocytosis and the activity of cell polarity proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Vesicular Transport / antagonists & inhibitors
  • Adaptor Proteins, Vesicular Transport / genetics
  • Adaptor Proteins, Vesicular Transport / metabolism*
  • Animals
  • Blotting, Western
  • Cell Adhesion Molecules / antagonists & inhibitors
  • Cell Adhesion Molecules / genetics
  • Cell Adhesion Molecules / metabolism*
  • Cell Movement
  • Cell Proliferation
  • Cells, Cultured
  • Chromatography, High Pressure Liquid
  • Endocytosis / physiology*
  • Endothelium, Vascular / cytology*
  • Endothelium, Vascular / metabolism
  • Ephrin-B2 / antagonists & inhibitors
  • Ephrin-B2 / genetics
  • Ephrin-B2 / metabolism*
  • Female
  • Humans
  • Immunoenzyme Techniques
  • Male
  • Mice
  • Mice, Transgenic
  • Morphogenesis
  • Neovascularization, Physiologic*
  • Phosphorylation
  • Protein Kinase C / genetics
  • Protein Kinase C / metabolism*
  • RNA, Messenger / genetics
  • RNA, Small Interfering / genetics
  • Rats
  • Real-Time Polymerase Chain Reaction
  • Receptors, Vascular Endothelial Growth Factor / antagonists & inhibitors
  • Receptors, Vascular Endothelial Growth Factor / genetics
  • Receptors, Vascular Endothelial Growth Factor / metabolism*
  • Retina / cytology
  • Retina / metabolism
  • Reverse Transcriptase Polymerase Chain Reaction
  • Signal Transduction
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Adaptor Proteins, Vesicular Transport
  • Cell Adhesion Molecules
  • Dab2 protein, mouse
  • Ephrin-B2
  • Pard3 protein, mouse
  • RNA, Messenger
  • RNA, Small Interfering
  • Receptors, Vascular Endothelial Growth Factor
  • PKC-3 protein
  • Protein Kinase C