Niche-less maintenance of HSCs by 2i

Cell Res. 2013 Apr;23(4):458-9. doi: 10.1038/cr.2013.14. Epub 2013 Jan 29.


Maintenance of hematopoietic stem cells (HSCs) in vitro has been believed to be difficult due to a lack of complete understanding of HSC quiescence maintained by the niche. Recent evidence suggests that in vitro maintenance of human and mouse long-term HSCs (LT-HSCs) is possible through dual inhibition (2i) of both GSK-3 and mTOR in the absence of cytokines, serum, or feeder cells.

Publication types

  • Comment

MeSH terms

  • Animals
  • Cell Proliferation / drug effects*
  • Hematopoietic Stem Cells / cytology*
  • Homeostasis / physiology*
  • Humans
  • TOR Serine-Threonine Kinases / metabolism*
  • Wnt Signaling Pathway / physiology*


  • TOR Serine-Threonine Kinases