Mineralisation of developmentally hypomineralised human enamel in vitro

Caries Res. 2013;47(3):259-63. doi: 10.1159/000346134. Epub 2013 Jan 29.


Molar-incisor hypomineralisation (MIH) is a problematic and costly condition. Caries remineralising agents are often recommended for MIH management despite the lack of evidence that these lesions have the capacity for increasing their mineral content. Following surface layer removal ± NaOCl pre-treatment and 14-day exposure to a CPP-ACFP solution at pH 5.5, MIH lesions were analysed using transverse microradiography and polarised light microscopy. Lesions were highly variable but treatment with the remineralising solution increased mineral content (1,828 ± 461 vol% min · µm, %R = 17.7 ± 5.7) and porosity decreased demonstrating the proof of concept that the mineral content of developmentally hypomineralised enamel can be improved after eruption.

MeSH terms

  • Caseins / therapeutic use*
  • Dental Enamel Hypoplasia / therapy*
  • Humans
  • In Vitro Techniques
  • Incisor / chemistry
  • Incisor / pathology
  • Microradiography
  • Molar / chemistry
  • Molar / pathology
  • Tooth Remineralization / methods*


  • Caseins
  • casein phosphopeptide-amorphous calcium phosphate nanocomplex