Chemotherapy-induced immunogenic modulation of tumor cells enhances killing by cytotoxic T lymphocytes and is distinct from immunogenic cell death

Int J Cancer. 2013 Aug 1;133(3):624-36. doi: 10.1002/ijc.28070. Epub 2013 Mar 16.

Abstract

Certain chemotherapeutic regimens trigger cancer cell death while inducing dendritic cell maturation and subsequent immune responses. However, chemotherapy-induced immunogenic cell death (ICD) has thus far been restricted to select agents. In contrast, several chemotherapeutic drugs modulate antitumor immune responses, despite not inducing classic ICD. In addition, in many cases tumor cells do not die after treatment. Here, using docetaxel, one of the most widely used cancer chemotherapeutic agents, as a model, we examined phenotypic and functional consequences of tumor cells that do not die from ICD. Docetaxel treatment of tumor cells did not induce ATP or high-mobility group box 1 (HMGB1) secretion, or cell death. However, calreticulin (CRT) exposure was observed in all cell lines examined after chemotherapy treatment. Killing by carcinoembryonic antigen (CEA), MUC-1, or PSA-specific CD8(+) CTLs was significantly enhanced after docetaxel treatment. This killing was associated with increases in components of antigen-processing machinery, and mediated largely by CRT membrane translocation, as determined by functional knockdown of CRT, PERK, or CRT-blocking peptide. A docetaxel-resistant cell line was selected (MDR-1(+), CD133(+)) by continuous exposure to docetaxel. These cells, while resistant to direct cytostatic effects of docetaxel, were not resistant to the chemomodulatory effects that resulted in enhancement of CTL killing. Here, we provide an operational definition of "immunogenic modulation," where exposure of tumor cells to nonlethal/sublethal doses of chemotherapy alters tumor phenotype to render the tumor more sensitive to CTL killing. These observations are distinct and complementary to ICD and highlight a mechanism whereby chemotherapy can be used in combination with immunotherapy.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Adenosine Triphosphate / biosynthesis
  • Antineoplastic Agents / pharmacology*
  • Apoptosis / drug effects*
  • Apoptosis / immunology
  • Breast Neoplasms / therapy
  • Calreticulin / analysis
  • Carcinoembryonic Antigen / immunology
  • Cell Line, Tumor
  • Colorectal Neoplasms / therapy
  • Cytotoxicity, Immunologic*
  • Dendritic Cells / immunology
  • Docetaxel
  • Female
  • HMGB1 Protein / biosynthesis
  • Humans
  • Immunotherapy
  • Male
  • Mucin-1 / immunology
  • Prostate-Specific Antigen / immunology
  • Prostatic Neoplasms / therapy
  • RNA Interference
  • RNA, Small Interfering
  • T-Lymphocytes, Cytotoxic / drug effects
  • T-Lymphocytes, Cytotoxic / immunology*
  • T-Lymphocytes, Cytotoxic / metabolism
  • Taxoids / pharmacology*
  • eIF-2 Kinase / genetics

Substances

  • Antineoplastic Agents
  • Calreticulin
  • Carcinoembryonic Antigen
  • HMGB1 Protein
  • MUC1 protein, human
  • Mucin-1
  • RNA, Small Interfering
  • Taxoids
  • Docetaxel
  • Adenosine Triphosphate
  • PERK kinase
  • eIF-2 Kinase
  • Prostate-Specific Antigen