Optimization extraction of Ganoderma lucidum polysaccharides and its immunity and antioxidant activities

Int J Biol Macromol. 2013 Apr:55:301-6. doi: 10.1016/j.ijbiomac.2013.01.022. Epub 2013 Jan 29.


Extraction of Ganoderma lucidum polysaccharides (GLP) was optimized by response surface method (RSM). By running the optimization program with design expert within the experimental range investigated, the following optimum values were obtained: extraction time 230 min; extraction temperature 95 °C, and extraction number 5. The predicted polysaccharides production was 1.45%. Results showed that GLP significantly reduced the levels of serum IL-6 and TNF-α levels and increased the levels of serum IL-2, IL-4 and IL-10 in GLP-treated rats compared to gastric cancer model rats. In addition, administration of Ganoderma lucidum polysaccharides to GLP-treated group of rats improved the levels of serum and gastric tissue SOD, CAT and GSH-Px toward the control values in a dose-dependent manner. These findings show that GLP can enhance immunity and antioxidant activities in gastric cancer rats.

MeSH terms

  • Animals
  • Antioxidants / chemistry
  • Antioxidants / pharmacology*
  • Fungal Polysaccharides / chemistry
  • Fungal Polysaccharides / immunology*
  • Fungal Polysaccharides / pharmacology*
  • Gastric Mucosa / metabolism
  • Glutathione / metabolism
  • Inflammation Mediators / metabolism
  • Interleukin-2 / biosynthesis
  • Lymphocyte Activation / drug effects
  • Lymphocyte Activation / immunology
  • Lymphocytes / drug effects
  • Lymphocytes / immunology
  • Lymphocytes / metabolism
  • Male
  • Malondialdehyde / metabolism
  • Rats
  • Reishi / chemistry*
  • Stomach / drug effects
  • Stomach / immunology


  • Antioxidants
  • Fungal Polysaccharides
  • Inflammation Mediators
  • Interleukin-2
  • Malondialdehyde
  • Glutathione