A modified superantigen rescues Ly6G- CD11b+ blood monocyte suppressor function and suppresses antigen-specific inflammation in EAE

Autoimmunity. 2013 Jun;46(4):269-78. doi: 10.3109/08916934.2013.767893.


In a previous study, we showed that the Ly6G(-)CD11b(+) blood monocytes residing in naïve mice are intrinsically immunosuppressive and that loss of this suppressive function may contribute to the development of autoimmunity in experimental autoimmune encephalomyelitis (EAE), a murine model of human multiple sclerosis. Here we report that mice treated with a modified superantigen coupled to myelin oligodendrocyte glycoprotein 35-55 (MOG(35-55)) peptide (DM-MOG(35-55)) suppressed the development of EAE. The treatment was associated with impaired MOG(35-55)-specific T cell proliferation and a decrease in IL-17 and IFNγ production in the draining lymph nodes. Analysis of circulating blood immune cells showed that the suppressor function of Ly6G(-)CD11b(+) blood monocytes was reduced in EAE mice, but was restored in mice treated with DM-MOG(35-55). Importantly, adoptive transfer of blood CD11b(+)Ly6G(-) cells isolated from DM-MOG(35-55)-treated mice protected recipient mice from developing EAE. Together, these results show that DM coupled to the auto-antigen MOG(35-55): 1) suppresses EAE via antigen-specific suppression of T cell responses, and 2) re-establishes suppressor function of Ly6G(-)CD11b(+) blood monocytes. Auto-antigens coupled to DM could therefore represent a new therapeutic approach for controlling inappropriate inflammation in autoimmune diseases such as multiple sclerosis by inducing antigen-specific T cell suppression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adoptive Transfer
  • Animals
  • Antigens, Ly / metabolism
  • Bacterial Toxins / chemistry
  • Bacterial Toxins / immunology
  • CD11b Antigen / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / immunology*
  • Encephalomyelitis, Autoimmune, Experimental / metabolism
  • Encephalomyelitis, Autoimmune, Experimental / therapy
  • Epitopes / immunology*
  • Exotoxins / chemistry
  • Exotoxins / immunology
  • Lymphocyte Activation / immunology
  • Mice
  • Monocytes / immunology*
  • Monocytes / metabolism
  • Myelin-Oligodendrocyte Glycoprotein / chemistry
  • Myelin-Oligodendrocyte Glycoprotein / immunology
  • Peptide Fragments / chemistry
  • Peptide Fragments / immunology
  • Superantigens / immunology*
  • T-Lymphocyte Subsets / immunology


  • Antigens, Ly
  • Bacterial Toxins
  • CD11b Antigen
  • Epitopes
  • Exotoxins
  • Ly6G antigen, mouse
  • Myelin-Oligodendrocyte Glycoprotein
  • Peptide Fragments
  • Superantigens
  • mitogenic exotoxin Z protein, Streptococcus
  • myelin oligodendrocyte glycoprotein (35-55)