[Standardizing a central nervous system tumor DNA biobank]

Cir Cir. Jul-Aug 2012;80(4):327-32.
[Article in Spanish]

Abstract

Background: Brain tumors are one of the leading cancers worldwide; in the National Institute of Neurology and Neurosurgery (INNN) these tumors are the leading cause of morbitity and mortality.

Objective: Standardize biopsies, colletion, processing and storage biologic material of molecular studies.

Methods: with a previously signed surgical consent, a tumor and blood biopsy was done to 134 patients. Their DNA was extracted and a database was filled considering technical, ethical and legal aspects. In order to have optimal biologic material the procedure was standardized between the surgical and research laboratory teams.

Results: The biopsy, transportation, processing and storage were standardized. 134 patients were included (67 male and 67 female) with an average age of 46.28 years (range 15-81). The most frequently biopsied tumor was the meningioma (42%). The integrity of the obtained material was determined by agarose gel electrophoretic analysis.

Conclusion: the INNN biobank has a standardized system that biopsies, processes and stores optimum quality biologic material that will be the basis of future molecular studies.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • Aged, 80 and over
  • Biological Specimen Banks / organization & administration
  • Biological Specimen Banks / standards*
  • Biopsy / standards
  • Central Nervous System / chemistry
  • Central Nervous System / pathology
  • Central Nervous System Neoplasms / chemistry
  • Central Nervous System Neoplasms / genetics
  • Central Nervous System Neoplasms / pathology*
  • Central Nervous System Neoplasms / secondary
  • Cranial Nerve Neoplasms / chemistry
  • Cranial Nerve Neoplasms / genetics
  • Cranial Nerve Neoplasms / pathology
  • DNA, Neoplasm* / analysis
  • DNA, Neoplasm* / genetics
  • DNA, Neoplasm* / isolation & purification
  • Databases, Factual
  • Electrophoresis, Agar Gel
  • Female
  • Humans
  • Male
  • Meningioma / chemistry
  • Meningioma / genetics
  • Meningioma / pathology*
  • Mexico
  • Middle Aged
  • Neoplasms, Neuroepithelial / chemistry
  • Neoplasms, Neuroepithelial / genetics
  • Neoplasms, Neuroepithelial / pathology*
  • Peripheral Nervous System Neoplasms / chemistry
  • Peripheral Nervous System Neoplasms / genetics
  • Peripheral Nervous System Neoplasms / pathology
  • Preservation, Biological / methods
  • Preservation, Biological / standards
  • Quality Assurance, Health Care
  • Specimen Handling / standards
  • Spinal Nerves / chemistry
  • Spinal Nerves / pathology
  • Transportation / standards
  • Young Adult

Substances

  • DNA, Neoplasm