Differential dose effect of fish oil on inflammation and adipose tissue gene expression in chronic kidney disease patients

Nutrition. 2013 May;29(5):730-6. doi: 10.1016/j.nut.2012.10.011. Epub 2013 Jan 30.

Abstract

Objective: The beneficial effects of ω-3 polyunsaturated fatty acids (PUFAs) in cardiovascular disease are partly attributed to their anti-inflammatory properties. Their potential effect on the adipose tissue of chronic kidney disease (CKD) patients has never been explored.

Methods: To determine the metabolic effect of supplementation with two different doses of fish oil (FO), 12 non-dialyzed patients with stage IV/V CKD were randomly allocated to receive 1.8 g or 3.6 g/d of ω-3 PUFA for 10 wk. Metabolic parameters, adipose tissue function, and gene expression were evaluated at baseline and 10 wk.

Results: Body weight, fat mass, energy intake, fasting glucose, and insulin were unchanged. The daily intake of 3.6 g of ω-3 PUFA resulted in decreased serum triacylglycerol and increased high-density lipoprotein cholesterol, whereas low-density lipoprotein cholesterol increased with 1.8 g of ω-3 PUFA. Serum adiponectin, leptin, C-reactive protein, and tumor necrosis factor-α were not modified in either group. Interleukin-6 levels tended to decrease with 1.8 g of ω-3 PUFA. Additionally, a subset of inflammation-related genes (CD68 and MMP9) was reduced in subcutaneous adipose tissue in this group. Adiponectin, leptin, and adipoR2 gene expression were upregulated with 3.6 g of ω-3 PUFA.

Conclusions: A moderate dose of FO alters the gene expression profile of adipose tissue to a more antiinflammatory status. Higher doses of FO have a favorable effect on lipid profile and lead to the upregulation of adipokines gene expression suggesting a different dose response to ω-3 PUFA administration in patients with CKD.

Publication types

  • Comparative Study
  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipokines / genetics
  • Adipokines / metabolism
  • Adipose Tissue / drug effects*
  • Adipose Tissue / metabolism
  • Adult
  • Antigens, CD / genetics
  • Antigens, CD / metabolism
  • Antigens, Differentiation, Myelomonocytic / genetics
  • Antigens, Differentiation, Myelomonocytic / metabolism
  • Body Weight / drug effects
  • Dose-Response Relationship, Drug
  • Energy Intake / drug effects
  • Fatty Acids, Omega-3 / administration & dosage
  • Fatty Acids, Omega-3 / pharmacology
  • Fatty Acids, Omega-3 / therapeutic use
  • Female
  • Fish Oils / administration & dosage*
  • Fish Oils / pharmacology
  • Fish Oils / therapeutic use
  • Gene Expression / drug effects*
  • Humans
  • Inflammation / drug therapy*
  • Inflammation / genetics
  • Inflammation / metabolism
  • Inflammation Mediators / metabolism*
  • Interleukin-5 / blood
  • Lipids / blood*
  • Male
  • Matrix Metalloproteinase 9 / genetics
  • Matrix Metalloproteinase 9 / metabolism
  • Middle Aged
  • Receptors, Adiponectin / genetics
  • Receptors, Adiponectin / metabolism
  • Renal Insufficiency, Chronic / drug therapy*
  • Renal Insufficiency, Chronic / genetics
  • Renal Insufficiency, Chronic / metabolism
  • Subcutaneous Fat / drug effects
  • Subcutaneous Fat / metabolism
  • Up-Regulation

Substances

  • ADIPOR2 protein, human
  • Adipokines
  • Antigens, CD
  • Antigens, Differentiation, Myelomonocytic
  • CD68 antigen, human
  • Fatty Acids, Omega-3
  • Fish Oils
  • Inflammation Mediators
  • Interleukin-5
  • Lipids
  • Receptors, Adiponectin
  • Matrix Metalloproteinase 9