Mapping of the binding sites involved in PSP94-CRISP-3 interaction by molecular dissection of the complex

Biochim Biophys Acta. 2013 Apr;1830(4):3019-29. doi: 10.1016/j.bbagen.2013.01.015. Epub 2013 Jan 31.


Background: Human Prostate Secretory Protein of 94 amino acids (PSP94) has been shown to bind human CRISP-3 (cysteine-rich secretory protein 3) with very high affinity. CRISP-3 belongs to the CRISP family of proteins having a PR-1 (pathogenesis related protein 1) domain at its N-terminal and ion channel regulatory (ICR) domain at its C-terminal connected by a hinge region. Functional significance of this complex is not yet known.

Methods: In order to identify the residues and/or regions involved in PSP94-CRISP-3 interaction, site-directed mutagenesis was employed. Effect of the mutations on the interaction was studied by co-immunoprecipitation (Co-IP).

Results: For PSP94, amino acids Y(3), F(4), P(56) and the C-terminal β-strand were found to be crucial for interacting with CRISP-3. A disulfide bond between the two domains of PSP94 (C(37)A-C(73)A) was also important for this interaction. In case of CRISP-3, the N-terminal domain alone could not maintain a strong interaction with PSP94 but it required presence of the hinge region and not the C-terminal domain. Apart from CRISP-3, CRISP-2 was also found to interact with human PSP94. Based on our findings the most likely model of PSP94-CRISP-3 complex has been proposed.

Conclusion: The terminal β-strands of PSP94 contact the first α-helix and the hinge region of CRISP-3.

General significance: Involvement of the hinge region of CRISPs in interaction with PSP94 may affect the domain movement of CRISPs essential for the ion-channel regulatory activity resulting in inhibition of this activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Binding Sites
  • Cell Line, Tumor
  • Humans
  • Male
  • Models, Molecular
  • Molecular Sequence Data
  • Prostatic Secretory Proteins / chemistry*
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Salivary Proteins and Peptides / chemistry*
  • Seminal Plasma Proteins / chemistry*


  • CRISP3 protein, human
  • Prostatic Secretory Proteins
  • Salivary Proteins and Peptides
  • Seminal Plasma Proteins
  • beta-microseminoprotein