In vivo dynamic process imaging using real-time optical-resolution photoacoustic microscopy

J Biomed Opt. 2013 Feb;18(2):26001. doi: 10.1117/1.JBO.18.2.026001.


The authors demonstrate in vivo dynamic process imaging using a label-free real-time optical-resolution photoacoustic microscope (OR-PAM). This reflection-mode system takes advantage of a 532-nm fiber laser source with a high pulse repetition rate of up to 600 kHz combined with a fast-scanning mirror system. Microvasculature in SCID mouse ears is imaged at near real-time (0.5 fps) for a 1×1 mm2 field of view (FOV) with micron-scale lateral resolution. We also demonstrate imaging of cardiac-induced microhemodynamics in murine microvasculature at real-time frame-rates (30 fps) over a 250×250 μm2 FOV using real-time C-scan OR-PAM with ability to provide sustained imaging with near real-time feedback for focusing and positioning.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Computer Systems
  • Ear / blood supply
  • Hemodynamics
  • Lasers, Solid-State
  • Mice
  • Mice, SCID
  • Microscopy, Acoustic / instrumentation
  • Microscopy, Acoustic / methods*
  • Microvessels / anatomy & histology
  • Microvessels / physiology
  • Optical Devices
  • Optical Phenomena
  • Photoacoustic Techniques / instrumentation
  • Photoacoustic Techniques / methods*