The therapeutic applications of celery oil seed extract on the plasticizer di(2-ethylhexyl) phthalate toxicity

Toxicol Ind Health. 2015 Apr;31(4):355-66. doi: 10.1177/0748233713475515. Epub 2013 Feb 1.


The present study investigated the impact of two doses, 500 mg/kg and 1000 mg/kg, of di(2-ethylhexyl) phthalate (DEHP) and studied the possible therapeutic dose of celery oil seed extract for 6 weeks on some atheroscelerogenic, obesogenic, antioxidant and liver functions in rats. Both doses of DEHP caused over-expression of peroxisome proliferator-activated receptor alpha (PPARα) messenger RNA with significant increase in liver weights, relative liver weights, serum cholesterol (Chol), triglycerides, low-density lipoprotein Chol, liver total lipids, along with an increase in the activities of serum aspartate aminotransferase, alanine aminotransferase, serum endothelin 1 and liver tissue thiobarbituric acid reactive substances (TBARS). Additionally, DEHP administration to rats resulted in significant decrease in final body weights, serum total protein, albumin, liver total protein and serum total nitric oxide. Our study confirmed the role of oral combination of Apium graveolens (celery) oil seed extract at small cumulative doses (50 µl/kg for 6 weeks) with DEHP in ameliorating the toxicological effects of DEHP, which was revealed in reducing the expression of PPARα, lipid profile, with restoring liver functions, vascular oxidative stress and inhibition of TBARS activity.

Keywords: Apium graveolens (celery oil); PPARα; di(2-ethylhexyl) phthalate; endothelin 1; obesity.

MeSH terms

  • Animals
  • Apium / chemistry*
  • Body Weight / drug effects
  • Drug Interactions
  • Lipids / blood
  • Liver / drug effects
  • Male
  • Organ Size / drug effects
  • PPAR alpha / genetics
  • PPAR alpha / metabolism
  • Phthalic Acids / toxicity*
  • Plant Oils / chemistry
  • Plant Oils / pharmacology*
  • Plasticizers / toxicity*
  • Polymerase Chain Reaction
  • Protective Agents / pharmacology*
  • Rats
  • Seeds / chemistry


  • Lipids
  • PPAR alpha
  • Phthalic Acids
  • Plant Oils
  • Plasticizers
  • Protective Agents