Euryhalocaulis caribicus gen. nov., sp. nov., a new member [corrected] of the family Hyphomonadaceae isolated from the Caribbean Sea

Curr Microbiol. 2013 Jun;66(6):606-12. doi: 10.1007/s00284-013-0314-9. Epub 2013 Feb 3.

Abstract

A new aerobic, Gram-negative, chemo-organotrophic, euryhaline bacterium, designated strain JL2009(T), was isolated from surface water of the Caribbean Sea. The strain formed flaxen colonies on marine ager 2216 (MA; Difco) medium. Cells were dimorphic, with stalks or a polar flagellum, and reproduction occurred by means of binary fission. Growth occurred at 15-45 °C (optimum at 35 °C), 0-15 % (w/v) NaCl (optimum at 1-10 %) and pH 5-9 (optimum at pH 7-8). Phylogenetic analysis based on 16S rRNA gene sequences showed that the strain formed a distinct evolutionary lineage within the family Hyphomonadaceae. Strain JL2009(T) was most closely related to Maricaulis parjimensis MCS 25(T) (92.2 % DNA sequence similarity), Woodsholea maritime CM2243(T) (90.9 %), and Oceanicaulis alexandrii C116-18(T) (90.9 %). The major respiratory quinone was Q-10. The dominant cellular fatty acids were summed feature 8 (C(18:1) ω7c), C(18:0) and 11-methyl C(18:1) ω7c. The polar lipid pattern indicated the presence of phospholipid, phosphatidyl glycerol and glycolipids. The G + C content of the genomic DNA was 70.5 mol%. On the basis of the chemotaxonomic and phenotypic characteristics and the phylogenetic evidence, strain JL2009(T) represents a novel genus and species in the family Hyphomonadaceae, for which the name Euryhalocaulis caribicus gen. nov., sp. nov. is proposed. The type strain of Euryhalocaulis caribicus is JL2009(T) (=CGMCC 1.12036(T) = JCM 18163(T)).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alphaproteobacteria / classification*
  • Alphaproteobacteria / genetics
  • Alphaproteobacteria / isolation & purification*
  • Bacterial Typing Techniques
  • Base Composition
  • Base Sequence
  • Caribbean Region
  • DNA, Bacterial / analysis
  • DNA, Bacterial / genetics
  • Fatty Acids / analysis
  • Molecular Sequence Data
  • Phylogeny
  • RNA, Ribosomal, 16S / genetics
  • Seawater / microbiology*
  • Sequence Analysis, DNA

Substances

  • DNA, Bacterial
  • Fatty Acids
  • RNA, Ribosomal, 16S

Associated data

  • GENBANK/JN798464