Abstract
Proteases are enzymes that cleave proteins. They occur widely in nature and play a fundamental role in cellular homeostasis; however, their presence can result in unwanted protein degradation during recombinant protein expression and purification. This unit introduces proteases, specifically focusing on the types commonly encountered during production of recombinant proteins. The strategies used to avoid and to prevent proteolysis are also described, with extensive consideration of the molecular, technical, and logistic methodologies involved.
MeSH terms
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Animals
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Escherichia coli / enzymology
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Escherichia coli Proteins / antagonists & inhibitors
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Escherichia coli Proteins / chemistry
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Humans
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Peptide Fragments / biosynthesis
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Peptide Fragments / chemistry
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Peptide Fragments / isolation & purification
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Peptide Hydrolases / chemistry*
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Protease Inhibitors / chemistry
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Protein Biosynthesis
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Protein Engineering
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Proteolysis*
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Recombinant Proteins / biosynthesis*
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Recombinant Proteins / chemistry
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Recombinant Proteins / isolation & purification
Substances
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Escherichia coli Proteins
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Peptide Fragments
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Protease Inhibitors
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Recombinant Proteins
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Peptide Hydrolases