An optical fiber-based uncaging system

Cold Spring Harb Protoc. 2013 Feb 1;2013(2):118-21. doi: 10.1101/pdb.top072900.

Abstract

This article describes the assembly and performance of a simple and inexpensive ultraviolet-flash system suitable for rapid focal photolysis of caged compounds in cultured neurons and brain slices. Advantages and limitations of this system are discussed. Examples are provided illustrating how this system can be used for stimulating neurons and mapping their functional inputs in brain slices.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Humans
  • Optical Fibers*
  • Ultraviolet Rays