Assessment of antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of pomegranate (Punica granatum) leaves

Food Chem Toxicol. 2013 May;55:470-5. doi: 10.1016/j.fct.2013.01.036. Epub 2013 Feb 1.


This study evaluated antioxidant, anti-inflammatory, anti-cholinesterase and cytotoxic activities of extracts with different polarities (hexane, dichloromethane, ethyl acetate, ethanol and methanol) obtained from Punica granatum leaves. Total phenolics (8.8-127.3mg gallic acid equivalent/g dry weight), flavonoids (1.2-76.9mg quercetin equivalent/g dry weight), tannins (63.7-260.8mg catechin equivalent/kg dry weight) and anthocyanins (0.41-3.73mg cyanidin-3-glucoside equivalent/g dry weight) of different extracts were evaluated. The methanolic extract presented a good IC50 by DPPH and ABTS assays (5.62 and 1.31mg/l respectively). The strongest 5-lipoxygenase (5-LOX), acetylcholinesterase (AChE) and butyrylcholinesterase (BuChE) inhibition activities were obtained for the ethanol extract (IC50 values of 6.20, 14.83 and 2.65mg/l, respectively) and the best cytotoxic activity against MCF-7 cells was obtained for the methanol extract (IC50=31mg/l). These important biological activities showed that P. granatum leaves could be a potential source of the active molecules intended for applications in pharmaceutical industry, but only after additional in vivo experiments.

MeSH terms

  • Anthocyanins / analysis
  • Anti-Inflammatory Agents / pharmacology*
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Antioxidants / pharmacology*
  • Cell Line, Tumor
  • Cholinesterase Inhibitors / pharmacology*
  • Flavonoids / analysis
  • Humans
  • Lythraceae / chemistry*
  • Phenols / analysis
  • Plant Extracts / pharmacology*
  • Plant Leaves / chemistry*
  • Tannins / analysis


  • Anthocyanins
  • Anti-Inflammatory Agents
  • Antineoplastic Agents, Phytogenic
  • Antioxidants
  • Cholinesterase Inhibitors
  • Flavonoids
  • Phenols
  • Plant Extracts
  • Tannins