Directed evolution of a synthetic RNA-protein module to create a new translational switch

Chem Commun (Camb). 2013 May 10;49(37):3833-5. doi: 10.1039/c3cc38688k.


A synthetic RNP-binding module was developed to produce an alternative translational switch: an in vitro selection experiment targeting a derivative of L7Ae protein (L7KK) identified a new H23 RNA aptamer that binds tightly to both L7KK and L7Ae. The switch serves as a translational OFF switch for constructing a NOR gate.

MeSH terms

  • Aptamers, Nucleotide / genetics
  • Aptamers, Nucleotide / metabolism*
  • Archaeal Proteins / genetics
  • Archaeal Proteins / metabolism*
  • Binding Sites
  • Electrophoretic Mobility Shift Assay
  • Genetic Engineering
  • HeLa Cells
  • Humans
  • Models, Molecular
  • Mutagenesis, Site-Directed
  • Nucleic Acid Conformation
  • Protein Binding
  • Protein Biosynthesis*
  • RNA, Archaeal / genetics
  • RNA, Archaeal / metabolism*


  • Aptamers, Nucleotide
  • Archaeal Proteins
  • RNA, Archaeal