Immobilization of bovine serum albumin-protected gold nanoclusters by using polyelectrolytes of opposite charges for the development of the reusable fluorescent Cu2+-sensor

Biosens Bioelectron. 2013 Jun 15;44:16-20. doi: 10.1016/j.bios.2013.01.005. Epub 2013 Jan 16.


In this paper, we reported a facile method of fabricating a reusable fluorescent Cu(2+)-sensor. To fabricate the reusable sensor, the bovine serum albumin-protected gold nanoclusters (BSAGNCs) were complexed with polyelectrolytes, i.e., positively charged polydiallyldimethylammonium (PDDA) and negatively charged polystyrenesulfonate (PSS), and were coated on a glass slide. The prepared film-modified glass slides exhibited the recyclability of fluorescent signal "off-on" behaviors: the fluorescence could be switched "off" by immersing the film sensors in Cu(2+) solution and "on" by washing with ethylene diamine tetraacetic acid (EDTA) solution. The prepared film-modified glass slides exhibited high selectivity towards Cu(2+) the fluorescence quenching behavior in the form of the Stern-Volmer equation. In addition, the spiked tap water samples were analyzed with satisfactory results. These demonstrations provide a new convenient approach to develop the BSAGNCs-based, reusable fluorescence sensors.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cations, Divalent / analysis
  • Cattle
  • Copper / analysis*
  • Fluorescent Dyes / chemistry*
  • Glass / chemistry*
  • Immobilized Proteins / chemistry*
  • Polymers / chemistry
  • Quaternary Ammonium Compounds / chemistry
  • Sensitivity and Specificity
  • Serum Albumin, Bovine / chemistry*
  • Spectrometry, Fluorescence / methods*
  • Sulfonic Acids / chemistry
  • Water / analysis


  • Cations, Divalent
  • Fluorescent Dyes
  • Immobilized Proteins
  • Polymers
  • Quaternary Ammonium Compounds
  • Sulfonic Acids
  • Water
  • Serum Albumin, Bovine
  • Copper