Evaluating amyloid Beta (aβ) 1-40 degradation by capillary electrophoresis

Methods Mol Biol. 2013;984:263-73. doi: 10.1007/978-1-62703-296-4_19.

Abstract

Here, we describe a capillary electrophoresis method for evaluating proteolysis of the amyloid beta peptide (Aβ) 1-40. This method is suitable for kinetic studies, demands little specialized equipment, and consumes only small quantities of a commercially available substrate whose physiological accumulation is thought to underlie the development of Alzheimer's disease.

MeSH terms

  • Amyloid beta-Peptides / chemistry*
  • Electrophoresis, Capillary / methods
  • Electrophoresis, Capillary / standards
  • Evaluation Studies as Topic
  • Humans
  • Insulysin / chemistry
  • Kinetics
  • Peptide Fragments / chemistry*
  • Proteolysis*
  • Reference Standards

Substances

  • Amyloid beta-Peptides
  • Peptide Fragments
  • amyloid beta-protein (1-40)
  • Insulysin