Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton

Ayuko Meguro; Takeo Tomita; Makoto Nishiyama; Tomohisa Kuzuyama

doi:10.1002/cbic.201200651

Identification and characterization of bacterial diterpene cyclases that synthesize the cembrane skeleton

Chembiochem. 2013 Feb 11;14(3):316-21. doi: 10.1002/cbic.201200651.

Authors

Ayuko Meguro¹, Takeo Tomita, Makoto Nishiyama, Tomohisa Kuzuyama

Affiliation

¹ Biotechnology Research Center, The University of Tokyo, 1-1-1 Yayoi, Tokyo 113-8657, Japan.

Abstract

Digging up skeletons: We report the identification and the functional characterization of two terpene cyclases (DtcycA and DtcycB) that were mined from the genome of Streptomyces sp. SANK 60404. DtcycA and DtcycB are novel bacterial diterpene cyclases for the synthesis of the cembrane skeleton.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alkyl and Aryl Transferases / chemistry
Alkyl and Aryl Transferases / metabolism*
Bacterial Proteins / chemistry
Bacterial Proteins / metabolism*
Cyclization
Diterpenes / chemistry
Diterpenes / metabolism*
Genome, Bacterial
Kinetics
Streptomyces / enzymology*
Streptomyces / genetics

Substances

Bacterial Proteins
Diterpenes
cembrane
Alkyl and Aryl Transferases