Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source

Jun Lim; So Yeong Park; Jung Yun Huang; Sung Mi Han; Hong-Tae Kim

doi:10.1063/1.4789362

Large-field high-contrast hard x-ray Zernike phase-contrast nano-imaging beamline at Pohang Light Source

Rev Sci Instrum. 2013 Jan;84(1):013707. doi: 10.1063/1.4789362.

Authors

Jun Lim¹, So Yeong Park, Jung Yun Huang, Sung Mi Han, Hong-Tae Kim

Affiliation

¹ Pohang Accelerator Laboratory, POSTECH, Pohang 790-784, South Korea. limjun@postech.ac.kr

PMID: 23387659
DOI: 10.1063/1.4789362

Abstract

We developed an off-axis-illuminated zone-plate-based hard x-ray Zernike phase-contrast microscope beamline at Pohang Light Source. Owing to condenser optics-free and off-axis illumination, a large field of view was achieved. The pinhole-type Zernike phase plate affords high-contrast images of a cell with minimal artifacts such as the shade-off and halo effects. The setup, including the optics and the alignment, is simple and easy, and allows faster and easier imaging of large bio-samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Equipment Design
Image Processing, Computer-Assisted
Light*
Macrophages / cytology
Microscopy, Phase-Contrast / instrumentation*
Nanotechnology / instrumentation*
Silicon Dioxide / chemistry
X-Rays

Substances

Silicon Dioxide