Effects of conditioned media from human amniotic epithelial cells on corneal alkali injuries in rabbits

J Vet Sci. 2013;14(1):61-7. doi: 10.4142/jvs.2013.14.1.61. Epub 2013 Feb 5.


This study was performed to evaluate the effects of conditioned media (CM) from human amniotic epithelial cells (HAECs) on the corneal wound healing process. Eighteen rabbits (36 eyes) were used and randomly assigned to three groups according treatment: CM from HAECs (group 1), vehicle alone (group 2), and saline (group 3). Corneal alkali injuries were induced with 1 N sodium hydroxide. Each reagent used for treatment evaluation was injected into the dorsal bulbar subconjunctiva and the area of the corneal epithelial defect was measured every other day. Two animals from each group were euthanized at a time on days 3, 7, and 15, and the cornea was removed for histological examination. The sum of the epithelial defect areas measured on day 0 to day 6 as well as day 0 to day 14 in group 1 was significantly smaller than those of other groups. Histological examination revealed that the group 1 corneas had less inflammatory cell infiltration and showed more intact epithelial features compared to the other groups. These results suggest that CM from HAECs promote corneal wound healing in rabbits.

Keywords: conditioned media; corneal alkali injury; human amniotic epithelial cell; rabbits.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkalies / toxicity*
  • Amnion / cytology*
  • Animals
  • Corneal Diseases / chemically induced
  • Corneal Diseases / therapy
  • Corneal Diseases / veterinary*
  • Corneal Injuries*
  • Culture Media, Conditioned / pharmacology*
  • Epithelial Cells / physiology*
  • Humans
  • Male
  • Rabbits*


  • Alkalies
  • Culture Media, Conditioned