Development of PCR assays for detection of Trichomonas vaginalis in urine specimens

J Clin Microbiol. 2013 Apr;51(4):1298-300. doi: 10.1128/JCM.03101-12. Epub 2013 Feb 6.


Trichomonas vaginalis infections are usually asymptomatic or can result in nonspecific clinical symptoms, which makes laboratory-based detection of this protozoan parasite essential for diagnosis and treatment. We report the development of a battery of highly sensitive and specific PCR assays for detection of T. vaginalis in urine, a noninvasive specimen, and development of a protocol for differentiating among Trichomonas species that commonly infect humans.

Publication types

  • Evaluation Study

MeSH terms

  • Adolescent
  • Child
  • Child, Preschool
  • Clinical Laboratory Techniques / methods*
  • Female
  • Humans
  • Infant
  • Molecular Diagnostic Techniques / methods*
  • Parasitology / methods*
  • Polymerase Chain Reaction / methods*
  • Sensitivity and Specificity
  • Trichomonas Infections / diagnosis*
  • Trichomonas vaginalis / classification
  • Trichomonas vaginalis / genetics
  • Trichomonas vaginalis / isolation & purification*
  • Urine / parasitology*