MicroRNA 181a influences the expression of HMGB1 and CD4 in acute Leukemias

Anticancer Res. 2013 Feb;33(2):445-52.


Dysregulation of microRNAs (miRs) has been linked to several types of cancer. In the present study, we investigated the expression of miR-181a in different leukemia cell lines and healthy hematopoietic cells, as well as its influence on cell proliferation, metabolic activity and potential targets. Expression of miR-181a differed between various leukemia cell lines and mature blood cells. Inhibition of miR 181a expression in T- and B-Acute Lymphoblastic Leukemia (ALL) cells revealed an influence on the potential targets High-Mobility-Group-Protein B1 (HMGB1) and Cluster of Differentiation 4 (CD4). Overexpression of miR-181a in AML cells led to a significant decrease in cell proliferation and metabolic activity. The present data indicate a possible role of this specific miRNA in immunogenicity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Western
  • CD4 Antigens / biosynthesis*
  • CD4 Antigens / genetics
  • Cell Line, Tumor
  • Cell Separation
  • Electroporation
  • Flow Cytometry
  • Gene Expression Profiling
  • Gene Expression Regulation / genetics*
  • HMGB1 Protein / biosynthesis*
  • HMGB1 Protein / genetics
  • Humans
  • Leukemia / genetics*
  • Leukemia / metabolism*
  • MicroRNAs / genetics*
  • Real-Time Polymerase Chain Reaction


  • CD4 Antigens
  • HMGB1 Protein
  • MIrn181 microRNA, human
  • MicroRNAs