Robust, synergistic regulation of human gene expression using TALE activators

Nat Methods. 2013 Mar;10(3):243-5. doi: 10.1038/nmeth.2366. Epub 2013 Feb 10.


Artificial activators designed using transcription activator-like effector (TALE) technology have broad utility, but previous studies suggest that these monomeric proteins often exhibit low activities. Here we demonstrate that TALE activators can robustly function individually or in synergistic combinations to increase expression of endogenous human genes over wide dynamic ranges. These findings will encourage applications of TALE activators for research and therapy, and guide design of monomeric TALE-based fusion proteins.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Binding Sites
  • Cell Culture Techniques
  • Fibroblasts / metabolism
  • Genetic Engineering / methods*
  • HEK293 Cells
  • Humans
  • MicroRNAs / genetics*
  • Plasmids
  • Promoter Regions, Genetic
  • Reverse Transcriptase Polymerase Chain Reaction
  • Tandem Repeat Sequences
  • Transcription Factors / genetics*
  • Transcriptional Activation*
  • Transfection
  • Vascular Endothelial Growth Factor A / genetics*
  • Xanthomonas
  • Zinc Fingers / genetics


  • MIRN302A microRNA, human
  • MIRN367 microRNA, human
  • MicroRNAs
  • Transcription Factors
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A