Prevention of neutrophil protein oxidation with vitamins C and E diet supplementation without affecting the adaptive response to exercise

Int J Sport Nutr Exerc Metab. 2013 Feb;23(1):31-9. doi: 10.1123/ijsnem.23.1.31.

Abstract

The authors studied the effects of antioxidant diet supplementation with an almond-based beverage on neutrophil antioxidants, nitrite, and protein oxidative alterations after exercise. Fourteen trained male amateur runners were randomly assigned in a double-blind fashion to receive antioxidant supplementation (152 mg/d vitamin C and 50 mg/d vitamin E) or placebo using an almond-based beverage for 1 mo and participated in a half-marathon race. Blood samples were taken before and after the half-marathon and after 3 hr recovery. Supplementation significantly increased basal neutrophil vitamin C compared with placebo (p < .05). Exercise increased neutrophil vitamin E levels in the supplemented group and decreased vitamin C in both groups after recovery (p < .05). Neutrophil catalase and glutathione peroxidase gene expression and nitrite levels were significantly increased as result of exercise (p < .05). Nitrotyrosine and protein carbonyl derivates increased only in the placebo group after exercise (p < .05), and these values remained high at recovery. No significant differences were evidenced in caspase-3 activity and DNA damage. Antioxidant supplementation with vitamins C and E reduced the exercise-induced oxidation of proteins in neutrophils, without altering the antioxidant adaptive response, as evidenced by the increased catalase and glutathione peroxidase gene expression.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Antioxidants / pharmacology*
  • Ascorbic Acid / pharmacology*
  • Caspase 3 / metabolism
  • DNA Damage
  • Dietary Supplements*
  • Double-Blind Method
  • Glutathione Peroxidase / genetics
  • Glutathione Peroxidase / metabolism
  • Humans
  • Male
  • Middle Aged
  • Neutrophils / metabolism*
  • Nitrites / blood
  • Oxidation-Reduction
  • Oxidative Stress / drug effects*
  • Protein Carbonylation / drug effects
  • Running / physiology*
  • Vitamin E / pharmacology*
  • Vitamins / pharmacology

Substances

  • Antioxidants
  • Nitrites
  • Vitamins
  • Vitamin E
  • Glutathione Peroxidase
  • Caspase 3
  • Ascorbic Acid