Barcode DNA high-resolution melting (Bar-HRM) analysis as a novel close-tubed and accurate tool for olive oil forensic use

J Sci Food Agric. 2013 Jul;93(9):2281-6. doi: 10.1002/jsfa.6040. Epub 2013 Feb 11.


Background: The adulteration of high-priced olive oil with low-cost oils and the fraudulent labelling of oil products make the identification and traceability of vegetable oil species in the food chain very important. This paper describes a high-resolution melting analysis-based method using chloroplast barcoding regions as target (Bar-HRM) to obtain barcoding information for the major vegetable oil species and to quantitatively identify the botanical origin of plant oils. The detection of adulteration of olive oil with canola oil was used as a case study.

Results: The proposed method was capable of distinguishing among different vegetable oil species and detecting a level of 1% (w/w) of canola oil in olive oil.

Conclusion: Bar-HRM analysis is a more accurate, faster and less costly alternative method to authenticate vegetable oils, including olive oil, and to detect mixtures of oils.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Brassica napus / chemistry
  • Brassica napus / metabolism
  • DNA Barcoding, Taxonomic*
  • DNA, Plant / analysis*
  • DNA, Plant / metabolism
  • Fatty Acids, Monounsaturated / analysis
  • Fatty Acids, Monounsaturated / chemistry
  • Food Contamination
  • Food Inspection / methods*
  • Food Labeling*
  • Forensic Toxicology / methods
  • Fruit / chemistry*
  • Fruit / metabolism
  • Greece
  • Limit of Detection
  • Nucleic Acid Denaturation
  • Olea / chemistry*
  • Olea / metabolism
  • Olive Oil
  • Plant Oils / chemistry*
  • Plant Proteins / genetics
  • Plant Proteins / metabolism
  • Rapeseed Oil
  • Real-Time Polymerase Chain Reaction
  • Ribulose-Bisphosphate Carboxylase / genetics
  • Ribulose-Bisphosphate Carboxylase / metabolism


  • DNA, Plant
  • Fatty Acids, Monounsaturated
  • Olive Oil
  • Plant Oils
  • Plant Proteins
  • Rapeseed Oil
  • RbcL protein, plastid
  • Ribulose-Bisphosphate Carboxylase