Use of surface plasmon resonance to study the adsorption of detergents on poly(dimethylsiloxane) surfaces

Electrophoresis. 2013 Apr;34(8):1249-52. doi: 10.1002/elps.201200573. Epub 2013 Mar 25.


This paper demonstrates the use of surface plasmon resonance to study adsorption (either reversible or irreversible) of detergents on PDMS surfaces in real time. The surface plasmon resonance measurements can directly provide information about the adsorption/desorption processes of detergents on the surface revealing the durability of the adsorbed layer and the anticipated degree of the EOF. Hydroxypropyl methylcellulose very strongly adsorbs onto PDMS and can be considered both a semipermanent layer and stable semipermanent coating. Adsorbed SDS or CTAB layers were stable for several minutes upon rinsing the surface with solution not containing the detergent. It was shown that SDS coated onto PDMS in microchips has the potential to afford similar separations in PDMS as found in conventional fused silica capillaries.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Adsorption
  • Cetrimonium
  • Cetrimonium Compounds / chemistry
  • Detergents / chemistry*
  • Dimethylpolysiloxanes / chemistry*
  • Electrophoresis, Capillary / instrumentation*
  • Silicon Dioxide / chemistry
  • Sodium Dodecyl Sulfate
  • Surface Plasmon Resonance / methods*


  • Cetrimonium Compounds
  • Detergents
  • Dimethylpolysiloxanes
  • Sodium Dodecyl Sulfate
  • baysilon
  • Silicon Dioxide
  • Cetrimonium