Effect of weight loss and exercise on angiogenic factors in the circulation and in adipose tissue in obese subjects

Obesity (Silver Spring). 2013 Mar;21(3):454-60. doi: 10.1002/oby.20060.

Abstract

Background: Vascular growth is a prerequisite for adipose tissue (AT) development and expansion. Some AT cytokines and hormones have effects on vascular development, like vascular endothelial growth factor (VEGF-A), angiopoietin (ANG-1), ANG-2 and angiopoietin-like protein-4 (ANGPTL-4).

Methods: In this study, the independent and combined effects of diet-induced weight loss and exercise on AT gene expression and proteins levels of those angiogenic factors were investigated. Seventy-nine obese males and females were randomized to: 1. Exercise-only (EXO; 12-weeks exercise without diet-restriction), 2. Hypocaloric diet (DIO; 8-weeks very low energy diet (VLED) + 4-weeks weight maintenance diet) and 3. Hypocaloric diet and exercise (DEX; 8-weeks VLED + 4-weeks weight maintenance diet combined with exercise throughout the 12 weeks). Blood samples and fat biopsies were taken before and after the intervention.

Results: Weight loss was 3.5 kg in the EXO group and 12.3 kg in the DIO and DEX groups. VEGF-A protein was non-significantly reduced in the weight loss groups. ANG-1 protein levels were significantly reduced 22-25% after all three interventions (P < 0.01). The ANG-1/ANG-2 ratio was also decreased in all three groups (P < 0.05) by 27-38%. ANGPTL-4 was increased in the EXO group (15%, P < 0.05) and 9% (P < 0.05) in the DIO group. VEGF-A, ANG-1, and ANGPTL-4 were all expressed in human AT, but only ANGPTL-4 was influenced by the interventions.

Conclusions: Our data show that serum VEGF-A, ANG-1, ANG-2, and ANGPTL-4 levels are influenced by weight changes, indicating the involvement of these factors in the obese state. Moreover, it was found that weight loss generally was associated with a reduced angiogenic activity in the circulation.

Publication types

  • Randomized Controlled Trial
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Adolescent
  • Adult
  • Angiogenesis Inducing Agents / blood*
  • Angiogenesis Inducing Agents / metabolism*
  • Angiopoietin-1 / blood
  • Angiopoietin-2 / blood
  • Angiopoietin-like 4 Protein
  • Angiopoietins / blood
  • Angiopoietins / genetics
  • Anthropometry
  • Diet, Reducing
  • Energy Intake
  • Exercise*
  • Female
  • Humans
  • Linear Models
  • Male
  • Middle Aged
  • Obesity / metabolism*
  • Vascular Endothelial Growth Factor A / blood
  • Weight Loss*
  • Young Adult

Substances

  • ANGPTL4 protein, human
  • Angiogenesis Inducing Agents
  • Angiopoietin-1
  • Angiopoietin-2
  • Angiopoietin-like 4 Protein
  • Angiopoietins
  • VEGFA protein, human
  • Vascular Endothelial Growth Factor A