Comparative study on hypocholesterolemic and antioxidant activities of various extracts of fenugreek seeds

Food Chem. 2013 Jun 1;138(2-3):1448-53. doi: 10.1016/j.foodchem.2012.11.003. Epub 2012 Nov 12.


The hypocholesterolemic and antioxidant activities of various extracts (water, methanol, ethyl acetate, hexane, dichloro-methane) of fenugreek seeds were investigated in cholesterol-fed rats. Only the ethyl acetate extract reduced total cholesterol, triglycerides, and low-density lipoprotein cholesterol (LDL-C) and increased high-density lipoprotein cholesterol (HDL-C) compared with those of rats fed a cholesterol-rich diet (HCD). Furthermore, the content of thiobarbituric acid-reactive substances (TBARS), and catalase and superoxide dismutase (SOD) in liver, heart and kidney decreased significantly after oral administration of the ethyl acetate extract, compared with those of HCD-fed rats. The phenolic and flavonoïd contents were highest in the methanol and the ethyl acetate extracts. These results showed that the ethyl acetate extract of the fenugreek seeds had a significant hypocholesterolemic effect and antioxidant activity in cholesterol-fed rats, whether this is partly due to the presence of flavonoïds in the extract needs further study.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Anticholesteremic Agents / administration & dosage*
  • Anticholesteremic Agents / chemistry
  • Antioxidants / administration & dosage*
  • Antioxidants / chemistry
  • Humans
  • Hypercholesterolemia / drug therapy*
  • Hypercholesterolemia / metabolism
  • Lipoproteins, LDL / metabolism
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Plant Extracts / administration & dosage*
  • Plant Extracts / chemistry
  • Rats
  • Rats, Wistar
  • Superoxide Dismutase / metabolism
  • Trigonella / chemistry*


  • Anticholesteremic Agents
  • Antioxidants
  • Lipoproteins, LDL
  • Plant Extracts
  • Superoxide Dismutase