Full-scale sequencing batch reactor (SBR) for domestic wastewater: performance and diversity of microbial communities

Bioresour Technol. 2013 Mar;132:262-8. doi: 10.1016/j.biortech.2013.01.027. Epub 2013 Jan 22.


This work describes the performance and microbial diversity in a sequencing batch reactor of a decentralized full-scale system for urban wastewater treatment under limited aeration. The removal efficiency was: 83% for soluble chemical oxygen demand (SCOD), 60% for N-NH4(+), 70% for total suspended solids (TSS) and 80% for volatile suspended solids (VSS). The biomass concentration had a maximum value around 8.7gVSSL(-1) for organic load rate of 0.6gCODL(-1)d(-1). The food/microorganism ratios showed average of 0.2gCOD/gVSSd. The sludge bacterial flocs were formed an irregular arrangement with organisms attached such as Euglypha sp. and pedunculate ciliates. It was observed the presence of Bacteria domains including Nitrosomonas spp., Nitrobacter spp., Nitrospira and C. "Accumulibacter" cluster. The DPAO activity was 70%. Denaturing gradient gel electrophoresis showed changes in ribotype number over biological treatment time among the groups observed being some are linked to nutrient removal. The reactor showed viability to treat domestic wastewater.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteria / genetics
  • Bacteria / metabolism*
  • Bacteria / ultrastructure
  • Biodiversity*
  • Biological Oxygen Demand Analysis
  • Biomass
  • Bioreactors*
  • Brazil
  • DNA, Ribosomal / genetics
  • Denaturing Gradient Gel Electrophoresis
  • Flocculation
  • In Situ Hybridization, Fluorescence
  • Microscopy, Electron, Scanning
  • Phosphorus / metabolism
  • Species Specificity
  • Waste Disposal, Fluid / methods*
  • Water Purification / methods*


  • DNA, Ribosomal
  • Phosphorus