Capillary microsampling and analysis of 4-µl blood, plasma and serum samples to determine human α-synuclein elimination rate in mice

Bioanalysis. 2013 Feb;5(4):449-62. doi: 10.4155/bio.12.337.


Background: The capillary microsampling technique was scaled down to enable repeated PK sampling of blood, plasma and serum from mice for the determination of the 14-kDa protein α-synuclein using the Gyrolab™ immunoassay platform.

Results: 4-µl plasma, serum or blood samples were taken from 36 mice, in total 648 samples were successfully collected and analyzed. Following intravenous administration of human α-synuclein to mice, the elimination of α-synuclein was rapid, with a half-life in plasma of 1.1 h. High endogenous levels in red blood cells in combination with some hemolysis led to a challenge in the evaluation of α-synuclein exposure in plasma and serum.

Conclusion: The small sample volumes and flexibility in choice of liquid matrices using the capillary microsampling technique enable repeated sampling in mouse studies, as well as multi-matrix analysis if needed. Liquid microsampling is well suited for micro- and nano-liter scale immunoassays.

MeSH terms

  • Animals
  • Capillaries / chemistry*
  • Female
  • Half-Life
  • Humans
  • Immunoassay / methods*
  • Mice
  • Mice, Inbred C57BL
  • Sample Size
  • alpha-Synuclein / blood*
  • alpha-Synuclein / pharmacokinetics*


  • SNCA protein, human
  • alpha-Synuclein