Predicting longevity in C. elegans: fertility, mobility and gene expression

Mech Ageing Dev. Jul-Aug 2013;134(7-8):291-7. doi: 10.1016/j.mad.2013.02.003. Epub 2013 Feb 13.

Abstract

Expression level of an hsp-16.2::gfp transgene is a predictor of longevity in Caenorhabditis elegans. Here we examine fertility, movement and longevity, comparing high-expressing ("bright") and low-expressing ("dim") animals. There was no differential fertility between bright and dim individuals, suggesting that dim worms were not excessively frail. Worms with high hsp-16.2::gfp expression had improved mobility, consistent with improved health span. We predicted that the increased longevity of the bright worms would be associated with increased expression of protective genes such as those shown to be upregulated in Age mutants. However, few genes were differentially transcribed, although internal controls (hsp-16.2 and family members) were differentially expressed. Quite surprising was the observation that expression level of the transgenic reporter was inherited by the progeny: in seven experiments bright worms consistently produced progeny that were brighter. We tested and ruled out possible artifacts such as differential copy-number of the transgene as an explanation of this differential brightness. These results suggest that a robust physiological state does not depend heavily upon transcriptional differences for its establishment, consistent with proteostatic mechanisms underlying the differential longevity.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Animals, Genetically Modified
  • Caenorhabditis elegans / genetics
  • Caenorhabditis elegans / metabolism*
  • Caenorhabditis elegans Proteins / biosynthesis
  • Caenorhabditis elegans Proteins / genetics
  • Fertility / genetics
  • Gene Expression Regulation*
  • Green Fluorescent Proteins / biosynthesis
  • Green Fluorescent Proteins / genetics
  • Heat-Shock Proteins / biosynthesis
  • Heat-Shock Proteins / genetics
  • Longevity*
  • Mutation
  • Transgenes

Substances

  • Caenorhabditis elegans Proteins
  • Heat-Shock Proteins
  • hsp-16.2 protein, C elegans
  • Green Fluorescent Proteins