Levels of PAH-DNA adducts in placental tissue and the risk of fetal neural tube defects in a Chinese population

Reprod Toxicol. 2013 Jun;37:70-5. doi: 10.1016/j.reprotox.2013.01.008. Epub 2013 Feb 15.

Abstract

We examined the relationship between PAH-DNA adduct levels in the placental tissue, measured by a highly sensitive (32)P-postlabeling assay, and the risk of fetal neural tube defects (NTDs). We further explored the interaction between PAH-DNA adducts and placental PAHs with respect to NTD risk. Placental tissues from 80 NTD-affected pregnancies and 50 uncomplicated normal pregnancies were included in this case-control study. Levels of PAH-DNA adducts were lower in the NTD group (8.12 per 10(8) nucleotides) compared to controls (9.92 per 10(8) nucleotides). PAH-DNA adduct concentrations below the median was associated with a 3-fold increased NTD risk. Women with a low PAH-DNA adduct level in concert with a high placental PAH level resulted in a 10-fold elevated risk of having an NTD-complicated pregnancy. A low level of placental PAH-DNA adducts was associated with an increased risk of NTDs; this risk increased dramatically when a low adduct level was coupled with a high placental PAH concentration.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adult
  • Asian Continental Ancestry Group
  • Case-Control Studies
  • DNA Adducts / metabolism*
  • Environmental Monitoring
  • Environmental Pollutants / analysis
  • Female
  • Humans
  • Neural Tube Defects / epidemiology*
  • Neural Tube Defects / metabolism
  • Placenta / chemistry
  • Placenta / metabolism*
  • Polycyclic Aromatic Hydrocarbons / analysis
  • Polycyclic Aromatic Hydrocarbons / metabolism*
  • Pregnancy
  • Risk Factors

Substances

  • DNA Adducts
  • Environmental Pollutants
  • Polycyclic Aromatic Hydrocarbons
  • polycyclic aromatic hydrocarbons-DNA adduct