Thiamine deficiency induces oxidative stress in brain mitochondria of Mus musculus

J Physiol Biochem. 2013 Sep;69(3):539-46. doi: 10.1007/s13105-013-0242-y. Epub 2013 Feb 17.


The present investigation evaluates the changes in the levels of antioxidant enzymes, lipid peroxidation (LPO), and protein carbonyl content (PCC) in brain mitochondria following thiamine deficiency (TD). The study was carried out on Mus musculus allocated into three groups, namely control and thiamine-deficient group for 8 (TD 8) and 10 (TD 10) days. The LPO was measured in terms of reduced glutathione (GSH) and thiobarbituric acid reactive substance (TBARS). Antioxidant enzymes such as superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) were measured biochemically. A significant increase in the TBARS (p < 0.0001) and PCC (p < 0.001) levels in group II (TD 8) and group III (TD 10) animals was observed in comparison to controls. The GSH levels were found to be reduced in both the treated groups compared to the control. A significant reduction in the activities of SOD was also observed in group II (p < 0.01) and group III (p < 0.0001) animals in comparison to the control. Enzymatic activities of CAT (p < 0.001) and GPx (p < 0.05) were found to be significantly reduced in group III (TD 10) in comparison to the control. In conclusion, reduction in the activities of antioxidant enzymes as well as an increase in LPO and PCC following TD implies oxidative stress in brain mitochondria that may further leads to neurodegeneration.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Brain / physiopathology
  • Catalase / metabolism
  • Glutathione / metabolism
  • Glutathione Peroxidase / metabolism
  • Lipid Peroxidation
  • Male
  • Mice
  • Mitochondria / metabolism*
  • Oxidative Stress
  • Protein Carbonylation
  • Superoxide Dismutase / metabolism
  • Thiamine / metabolism*
  • Thiamine Deficiency / metabolism*
  • Thiamine Deficiency / physiopathology
  • Thiobarbituric Acid Reactive Substances


  • Thiobarbituric Acid Reactive Substances
  • Catalase
  • Glutathione Peroxidase
  • Superoxide Dismutase
  • Glutathione
  • Thiamine