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, 335 (1), 201-4

Exosomes From Marrow Stromal Cells Expressing miR-146b Inhibit Glioma Growth

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Exosomes From Marrow Stromal Cells Expressing miR-146b Inhibit Glioma Growth

Mark Katakowski et al. Cancer Lett.

Abstract

Exosomes are 30-150 nm vesicles secreted by a wide range of mammalian cells that can contain microRNA (miRNA). To test if marrow stromal cell (MSC) exosomes could be used as a vehicle for delivery of anti-tumor miRNAs, we transfected MSCs with a miR-146b expression plasmid, and harvested exosomes released by the MSCs. Intra-tumor injection of exosomes derived from miR-146-expressing MSCs significantly reduced glioma xenograft growth in a rat model of primary brain tumor.

Conflict of interest statement

Conflict of Interest Statement

None

Figures

Figure 1
Figure 1
Exosomes from MSCs transfected with miR-146b or cel-miR-67 expression plasmids. A, Electron micrograph of MSC exosomes isolated from MSC culture medium. Scalebar = 500 nm. Data are mean ± sem.; comparison is two-tailed t-test. B, Western blot for beta;-actin, EGFR and NF-κB protein expression in 9L cells treated with M67-exo and M146-exo. C, Real-time PCR detection of miR-146b expression in M67-exo and M146-exo (n = 7), and in 9L cells treated with M67-exo or M146-exo (n = 3). D, Growth assay of primary cortical rat astrocytes treated with MSC exosomes, M67-exo or M146-exo. E, Growth assay of 9L glioma cells rat treated with MSC exosomes, M67-exo or M146-exo (*p = 0.027).
Figure 2
Figure 2
Intra-tumor injection of M146-exo reduced 9L glioma growth in rat brain. A, Representative H&E-stained coronal sections from rats sacrificed 10 days after tumor implantation. B, Volumetric measurement of 9L xenograft tumors 10 days after tumor implant, and 5 days after PBS, M67-exo, or M146-exo treatment (n = 8 per group). ANOVA: p = 0.042. Data are mean ± sem. Post hoc multiple comparisons are two-tailed t-tests.

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