A simple, rapid and inexpensive screening method for the identification of Pythium insidiosum

Juliana Simoni Moraes Tondolo; Erico Silva Loreto; Laura Bedin Denardi; Débora Alves Nunes Mario; Sydney Hartz Alves; Janio Morais Santurio

doi:10.1016/j.mimet.2013.02.002

A simple, rapid and inexpensive screening method for the identification of Pythium insidiosum

J Microbiol Methods. 2013 Apr;93(1):52-4. doi: 10.1016/j.mimet.2013.02.002. Epub 2013 Feb 16.

Authors

Juliana Simoni Moraes Tondolo¹, Erico Silva Loreto, Laura Bedin Denardi, Débora Alves Nunes Mario, Sydney Hartz Alves, Janio Morais Santurio

Affiliation

¹ Programa de Pós-Graduação em Farmacologia, Centro de Ciências da Saúde, Departamento de Microbiologia e Parasitologia, Universidade Federal de Santa Maria (UFSM), Av. Roraima no. 1000, Santa Maria, RS, Brazil. jutondolo@yahoo.com.br

PMID: 23419825
DOI: 10.1016/j.mimet.2013.02.002

Abstract

Growth of Pythium insidiosum mycelia around minocycline disks (30μg) did not occur within 7days of incubation at 35°C when the isolates were grown on Sabouraud, corn meal, Muller-Hinton or RPMI agar. This technique offers a simple and rapid method for the differentiation of P. insidiosum from true filamentous fungi.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Anti-Infective Agents / metabolism
Culture Media / chemistry
Humans
Mass Screening / methods*
Microbiological Techniques / methods*
Pythiosis / diagnosis
Pythium / drug effects
Pythium / growth & development
Pythium / isolation & purification*
Tetracycline / metabolism

Substances

Anti-Infective Agents
Culture Media
Tetracycline