Objective: To explore the effect of phytic acid on the human osteosarcoma U20S cells and its mechanisms in vitro.
Methods: MTT assay was used to examine the cell proliferation. Scanning electron microscope (SEM) was used to observe the surface ultrastructure of U20S cells. Hoechst 33342 fluorescence staining assay were applied to study the pro-apoptosis effects. The immune histochemisty method was used to detect c-myc expression.
Results: Phytic acid could significantly inhibit the growth of U20S. The surface ultrastructure of U20S cells showed distinct morphological changes corresponding to a typical cellular surface morphology of apoptosis. From fluorescence staining, we observed chromoplasm pyknosis and apoptotic body. Expression of c-myc protein decreased.
Conclusion: The anti-osteosarcoma mechanism of phytic acid may be related to its influence on inducing apoptosis and adjusting c-myc expression.