Presentation of viral antigen controlled by a gene in the major histocompatibility complex

Nature. 1990 May 31;345(6274):449-52. doi: 10.1038/345449a0.


We describe a mutant human cell line (LBL 721.174) that has lost a function required for presentation of intracellular viral antigens with class I molecules of the major histocompatibility complex (MHC), but retains the capacity to present defined epitopes as extracellular peptides. The cell also has a defect in the assembly and expression of class I MHC molecules, which we show can be restored by exposure of the cells to a peptide epitope. This phenotype suggests a defect in the association of intracellular antigen with class I molecules similar to that described for the murine mutant RMA-S (ref. 5), but in the present case the genetic defect can be mapped within the MHC locus on human chromosome 6.

MeSH terms

  • Antigen-Presenting Cells / physiology*
  • Antigens, Viral / immunology*
  • Cell Line
  • Chromosomes, Human, Pair 6*
  • Histocompatibility Antigens Class I / immunology
  • Histocompatibility Antigens Class I / metabolism*
  • Humans
  • Influenza A virus / immunology
  • Major Histocompatibility Complex*
  • Oligopeptides / metabolism
  • Precipitin Tests
  • Viral Matrix Proteins / immunology


  • Antigens, Viral
  • Histocompatibility Antigens Class I
  • Oligopeptides
  • Viral Matrix Proteins