Cryopreserved cord blood progenitors and their cell adhesion molecules are increased by coculture with osteoblasts and parathyroid hormone

J Pediatr Hematol Oncol. 2013 Aug;35(6):e229-33. doi: 10.1097/MPH.0b013e318282d64d.

Abstract

Despite advantages of cord blood (CB) cells, such as their high capacity for proliferation and low immunogenicity, CB transplantation is also associated with delayed neutrophil and platelet recovery relative to bone marrow transplantation. These limitations arise from the reduced abundances of primitive hematopoietic stem cells expressing adhesion molecules in CB relative to bone marrow. To address this limitation, we evaluated whether human parathyroid hormone (hPTH) could increase the number of primitive hematopoietic stem cells with adhesion molecules in cryopreserved CB. When cryopreserved CB cells were cocultured with differentiated osteoblasts in the presence of hPTH, numbers of CD34CD38 cells increased 4-fold after 7 days. Exposure to hPTH promoted clonogenic cell expansion and significantly increased the expression of adhesion molecules, such as CD44 (a cell surface glycoprotein) and VLA-4 (α4 integrin) in CD34 cells. This result shows that short-term coculture of cryopreserved CB with differentiated osteoblasts in the presence of hPTH may improve the rate of engraftment of CD34 cells through increasing the abundances of primitive cells bearing adhesion molecules.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Adhesion Molecules / biosynthesis*
  • Cell Differentiation
  • Coculture Techniques
  • Cord Blood Stem Cell Transplantation / methods
  • Cryopreservation
  • Fetal Blood / cytology*
  • Fetal Blood / metabolism
  • Hematopoietic Stem Cells / drug effects
  • Hematopoietic Stem Cells / metabolism*
  • Humans
  • Osteoblasts / cytology*
  • Parathyroid Hormone / metabolism*
  • Parathyroid Hormone / pharmacology
  • Real-Time Polymerase Chain Reaction
  • Reverse Transcriptase Polymerase Chain Reaction

Substances

  • Cell Adhesion Molecules
  • Parathyroid Hormone