Active protein and calcium hydroxyapatite bilayers grown by laser techniques for therapeutic applications

J Biomed Mater Res A. 2013 Sep;101(9):2706-11. doi: 10.1002/jbm.a.34572. Epub 2013 Feb 20.


Active protein and bioceramic calcium hydroxyapatite (HA) bilayers were grown by combining conventional pulsed laser deposition (PLD) and matrix-assisted pulsed laser evaporation (MAPLE) techniques. A pulsed UV KrF* excimer laser was used for the irradiations. The HA layers were grown by PLD. Proteins with antimicrobial action were attached to the bioceramic layers using MAPLE. The composite MAPLE targets were obtained by dissolving the proteins powder in distilled water. The crystalline status and chemical composition of the obtained structures were studied by X-ray diffractometry and Fourier transform infrared spectroscopy. The layers were grown for the design of advanced future metal implants coatings, ensuring both enhanced bone formation and localized antimicrobial therapy. Our results demonstrated that protein coatings improve bone cell proliferation in vitro. Immunofluorescence experiments show that actin filaments stretch throughout bone cells and sustain their optimal spreading.

Keywords: in vitro biocompatibility tests; laser techniques; protein and calcium hydroxyapatite bilayers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alloys
  • Biocompatible Materials / chemistry*
  • Cell Line
  • Cell Proliferation
  • Coated Materials, Biocompatible / chemistry
  • Durapatite / chemistry*
  • Humans
  • Lasers
  • Materials Testing
  • Muramidase / chemistry
  • Osseointegration
  • Osteoblasts / cytology
  • Papain / chemistry
  • Prostheses and Implants
  • Proteins / chemistry*
  • Surface Properties
  • Titanium


  • Alloys
  • Biocompatible Materials
  • Coated Materials, Biocompatible
  • Proteins
  • Durapatite
  • Titanium
  • Muramidase
  • Papain