An observational descriptive study to determine the frequency of Mycoplasma hominis, Ureaplasma parvum and Ureaplasma urealyticum isolates in patients with bacterial vaginosis was carried out in 296 patients who had vaginal secretion and were seen at two hospitals. The diagnosis was based on Amsel's criteria. Endocervical swabs were taken from women positive to this disease for M. hominis and Ureaplasma spp. diagnosis by traditional methods. Polymerase chain reaction identified U. parvum and U. urealyticum. Bacterial vaginosis was diagnosed in 30.1% of females, and in 77.5% of them the studied urogenital mycoplasmas were present. M. hominis was the most common species (71%) whereas U. parvum and Urealyticum were detected in 23.2 % and 5.8% of cases respectively. The diagnosis of Mycoplasmas and ureaplasmas should be performed in females with bacterial vaginosis, which will allow applying adequate therapeutic control and avoiding future pathologies in the genital tract.