Construction of ploidy series of Saccharomyces cerevisiae by the plasmid YCplac33-GHK

J Ind Microbiol Biotechnol. 2013 Apr;40(3-4):393-7. doi: 10.1007/s10295-013-1241-2. Epub 2013 Feb 22.


An effective approach, using the plasmid YCplac33-GHK, is developed to construct a ploidy series of Saccharomyces cerevisiae. YCplac33-GHK harbors the HO gene under the control of galactose-inducible promoter and KanMX4 as the selective marker. The simple method can solve the problem of industrial applications of strains with resistance genes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyribonucleases, Type II Site-Specific / genetics
  • Genes, Fungal
  • Plasmids / genetics*
  • Ploidies*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins / genetics


  • Saccharomyces cerevisiae Proteins
  • HO protein, S cerevisiae
  • Deoxyribonucleases, Type II Site-Specific