TALEN-mediated precise genome modification by homologous recombination in zebrafish

Nat Methods. 2013 Apr;10(4):329-31. doi: 10.1038/nmeth.2374. Epub 2013 Feb 24.


We report gene targeting via homologous recombination in zebrafish. We co-injected fertilized eggs with transcription activator-like effector nuclease mRNAs and a donor vector with long homologous arms targeting the tyrosine hydroxylase (th) locus, and we observed effective gene modification that was transmitted through the germ line. We also successfully targeted two additional genes. Homologous recombination in zebrafish with a dsDNA donor expands the utility of this model organism.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Base Sequence
  • Deoxyribonucleases / genetics
  • Deoxyribonucleases / metabolism*
  • Exons
  • Gene Expression Regulation, Developmental
  • Gene Expression Regulation, Enzymologic
  • Gene Targeting / methods
  • Genetic Engineering / methods
  • Genomics
  • RNA
  • Recombination, Genetic / physiology*
  • Tyrosine 3-Monooxygenase / genetics
  • Tyrosine 3-Monooxygenase / metabolism
  • Zebrafish / embryology
  • Zebrafish / genetics*


  • RNA
  • Tyrosine 3-Monooxygenase
  • Deoxyribonucleases