Neutron-encoded mass signatures for multiplexed proteome quantification

Nat Methods. 2013 Apr;10(4):332-4. doi: 10.1038/nmeth.2378. Epub 2013 Feb 24.


We describe a protein quantification method called neutron encoding that exploits the subtle mass differences caused by nuclear binding energy variation in stable isotopes. These mass differences are synthetically encoded into amino acids and incorporated into yeast and mouse proteins via metabolic labeling. Mass spectrometry analysis with high mass resolution (>200,000) reveals the isotopologue-embedded peptide signals, permitting quantification. Neutron encoding will enable highly multiplexed proteome analysis with excellent dynamic range and accuracy.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Carbon Isotopes
  • Chromatography, Liquid / methods*
  • Deuterium
  • Gene Expression Regulation, Fungal
  • Neutrons*
  • Nitrogen Isotopes
  • Protein Sorting Signals
  • Proteomics / methods*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / metabolism
  • Saccharomyces cerevisiae Proteins / chemistry*
  • Saccharomyces cerevisiae Proteins / genetics
  • Saccharomyces cerevisiae Proteins / metabolism
  • Tandem Mass Spectrometry / methods*


  • Carbon Isotopes
  • Nitrogen Isotopes
  • Protein Sorting Signals
  • Saccharomyces cerevisiae Proteins
  • Deuterium