Prenatal exposure to escitalopram and/or stress in rats: a prenatal stress model of maternal depression and its treatment

Psychopharmacology (Berl). 2013 Jul;228(2):231-41. doi: 10.1007/s00213-013-3030-z. Epub 2013 Feb 24.

Abstract

Rationale: A rigorously investigated model of stress and antidepressant administration during pregnancy is needed to evaluate possible effects on the mother.

Objective: The objective of this study was to develop a model of clinically relevant prenatal exposure to an antidepressant and stress during pregnancy to evaluate the effects on maternal care behavior.

Results: Female rats implanted with 28-day osmotic minipumps delivering the SSRI escitalopram throughout pregnancy had serum escitalopram concentrations in a clinically observed range (17-65 ng/ml). A separate cohort of pregnant females exposed to a chronic unpredictable mild stress paradigm on gestational days 10-20 showed elevated baseline (305 ng/ml), and acute stress-induced (463 ng/ml), plasma corticosterone concentrations compared to unstressed controls (109 ng/ml). A final cohort of pregnant dams were exposed to saline (control), escitalopram, stress, or stress and escitalopram to determine the effects on maternal care. Maternal behavior was continuously monitored over the first 10 days after parturition. A reduction of 35 % in maternal contact and 11 % in nursing behavior was observed due to stress during the light cycle. Licking and grooming behavior was unaffected by stress or drug exposure in either the light or dark cycle.

Conclusions: These data indicate that: (1) clinically relevant antidepressant treatment during human pregnancy can be modeled in rats using escitalopram; (2) chronic mild stress can be delivered in a manner that does not compromise fetal viability; and (3) neither of these prenatal treatments substantially altered maternal care post parturition.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Citalopram / administration & dosage
  • Citalopram / pharmacokinetics
  • Citalopram / pharmacology*
  • Corticosterone / blood
  • Darkness
  • Depression / complications
  • Depression / drug therapy*
  • Disease Models, Animal
  • Female
  • Infusion Pumps, Implantable
  • Light
  • Maternal Behavior
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley
  • Serotonin Uptake Inhibitors / administration & dosage
  • Serotonin Uptake Inhibitors / pharmacokinetics
  • Serotonin Uptake Inhibitors / pharmacology*
  • Stress, Psychological / physiopathology*

Substances

  • Serotonin Uptake Inhibitors
  • Citalopram
  • Corticosterone